GeneJect™

DNA, siRNA / miRNA transfection reagent.
Product Code:SizePriceQty
4100-01 100 µl
    54.96 €
ManualMSDSBrochure
4100-05 500 µl
    239.76 €
4100-15 1,5 ml
    599.76 €

Advantages of the product

  • Suitable for DNA, siRNA and miRNA transfection
  • Low cell toxicity
  • Efficient transfection of wide variety of primary cells and cell lines

Description

GeneJect™ is a transfection reagent, which is suitable for the delivery of plasmid DNA and short oligonucleotides, such as siRNAs as well as miRNA mimics and inhibitors.

GeneJect™ is less toxic when compared with lipofection. Toxicity is higher at low cell confluency.

GeneJect™ is efficient in the transfection of common cell lines of different origin, including HeLa, HEK293, PPC-1, HaCaT, A549, BEAS-2B, CCD19Lu, THP-1 and KOP (fetal bovine oropharynx cells). It also enables transfection of low level transfection cells including MDBK (Madin Darby Bovine Kidney cells).

GeneJect™ is efficient in the transfection of wide variety of primary cells, such as human primary keratinocytes, primary skin fibroblasts, melanocytes and monocytes.

100 μl of GeneJect™ allows for up to 50 transfections in 12-well tissue culture plates format.

 

DNA Transfection performance of GeneJect™ versus market top-standard transfection reagent

 

Figure 1. Uptake of GeneJect™ – plasmid DNA nanocomplexes

Cells were transfected with pEGFP plasmid DNA on a 12 well plate with 1 μg DNA and 2 μl Gene Ject™ per well and visualised under fluorescence microscope after 24h from transfection.

 HEKMDBK
GeneJect™
jetPRIME®

HEK - Easy to transfect Human embryonic kidney cells. MDBK - Hard to transfect Madin Darby bovine kidney cells.

 

miRNA Transfection performance of GeneJect™ versus market top-standard transfection reagent

 

Figure 2. Uptake of GeneJect™ – miRNA nanocomplexes

Dy547-labelled (red) miRNA mimics were transfected with either GeneJect™ or with a lipofectamine type of reagent siPORT-NeoFX at 30 nM concentration and stained after 24 h with DAPI (blue) and Alexa Fluor A488 Phalloidin (green).

 A549BEAS-2BCCD19-LuPPC-1
GeneJect™
siPORT
  Dy547 Phalloidin DAPI

A549 - Adenocarcinomic human alveolar basal epithelial cells, BEAS-2B - Human bronchial epithelial cell line, CCD19-Lu - Human lung fibroblast cells, PPC-1 - human prostatic cell line.

 

siRNA Transfection performance of GeneJect™

 

Figure 3. Inhibition of target gene (IRAK1) by GeneJect™ delivered miR-146a mimic

Cells were transfected either with control (cont) or miR-146a mimics at 30 nM concentration.

After 48 hours, a total RNA was purified and analyzed by RT-qPCR using primers specific to IRAK1. Relative expression compared to cont in each cell type (=1) is shown. Data represent mean ± SEM of four independent transfection. Student’s t-test, ** P < 0.01.

PPC-1 - Human prostatic cell line, A549 - Adenocarcinomic human alveolar basal epithelial cells, KC - Human primary keratinocytes, THP-1 - Human monocytic cell-line.

 

Figure 4. GeneJect™ efficiency in siRNA delivery

Human primary keratinocytes were transfected either with control (cont) or indicated specific siRNAs at 30 nM concentration using GeneJect™. After 48 hours, a total RNA was purified and analyzed by RT-qPCR using primers specific to IRAK1 and CARD10.

Relative expression compared to cont siRNA (=1) is shown. Data represent mean ± SEM of three independent transfection. Student’s t-test, * P < 0.05. ** P < 0.01.

GeneJect™