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  • 25/04/2024

In molecular biology research, a hugely significant stage is the effective conversion of RNA into complementary DNA (cDNA). It is precisely cDNA that forms the basis for many applications, from gene activity analysis to the creation of gene libraries. In this complex operation, a fundamental role is played by Transcriba, the Reverse Transcriptase derived from the Moloney Murine Leukemia Virus (M-MLV). This enzyme is incredibly versatile and capable of working with various types of genetic materials so there is no surprise it finds application in diverse research.

The applications of Transcriba are extensive:

  • In the construction of cDNA libraries, it plays a crucial role, facilitating the understanding of gene activity profiles and genome function.
  • During the synthesis of the first cDNA strand, Transcriba allows for the transformation of RNA into complementary DNA, opening the way for further analyses such as PCR, cloning, or sequencing.
  • In RT-qPCR tests, which are used for quantitative gene activity analysis, Transcriba initiates the reverse transcription of RNA into cDNA, which is then amplified and quantitatively assessed in real-time PCR reactions.