T4 DNA polymerase

Reaction buffer included. Form: solution, concentration 3 U/µl.
Product Code:SizePriceQty
1031-100 100 U
    30.96 €
ManualMSDS
1031-500 500 U
    119.76 €

Specification

 

form

solution

concentration

3 U/µl

unit definition

One unit of the enzyme catalyzes the incorporation of

10 nmol of deoxyribonucleotides into a polynucleotide

fraction in 30 min at 37 °C.

applications

  • Blunting of dsDNA ends by filling 5'-overhangs or removal of 3'-overhangs.
  • Blunting of PCR products containing 3'-dA overhangs (after amplification with Taq DNA polymerase)
  • Synthesis and labeling of DNA probes by the replacement reaction.
  • Oligonucleotide-directed site-specific mutagenesis.
  • Ligation-independent cloning of PCR products.

source

recombinant

storage buffer

100 mM KPO₄, 1 mM DTT, 50% Glycerol, pH 6.5 @ 25°C

Description

T4 DNA Polymerase is a template-dependent polymerase that catalyzes 5'-3' synthesis from primed single-stranded DNA. The enzyme also has 3'-5 'exonuclease activity. The T4 DNA polymerase has no 5'-3 'exonuclease activity.

The 3'-5 'exonuclease activity is much higher for ssDNA compared to dsDNA.

T4 DNA polymerase is active in most buffers used in PCR, reverse transcription, ligation buffer and in most buffers for restriction enzymes

T4 DNA polymerase