Exonuclease V (RecBCD)

Recombinant enzyme that cleaves linear single-stranded and double-stranded DNA in both 3' to 5' and 5' to 3' directions. Concentration 10 U/µl.
Product Code:SizePriceQty
1036-1000 1000 U
    83.28 €
ManualMSDS
1036-5000 5000 U
    332.64 €

Advantages of the product

  • DNA specific exonuclease that has ssDNA and dsDNA exonuclease activity and ATP-dependent ssDNA endonuclease activity.
  • Cleaves ssDNA and dsDNA in both 3' to 5' and 5' to 3' directions.
  • Degrades linear ssDNA and dsDNA leaving intact supercoiled and circular dsDNA.

Description

Exonuclease V is a complex of recombinant RecB, RecC and RecD proteins from E. coli. Enzyme has a helicase and nuclease activity. In the presence of ATP catalyzes exonucleolytic cleavage in both 3' to 5' and 5' to 3' directions to yield 5' phosphooligonucleotides.

Degradation of linear DNA by Exonuclease V (RecBCD) A&A compared to a competitor enzyme.

Exonuclease V (RecBCD)