Exonuclease V (RecBCD)
Recombinant enzyme that cleaves linear single-stranded and double-stranded DNA in both 3' to 5' and 5' to 3' directions. Concentration 10 U/µl.
| Product Code | Size | |
|---|---|---|
| 1036-1000 | 1000 U | On request. Contact: orders@aabiot.com |
| 1036-5000 | 5000 U | On request. Contact: orders@aabiot.com |
Advantages of the product
- DNA specific exonuclease that has ssDNA and dsDNA exonuclease activity and ATP-dependent ssDNA endonuclease activity.
- Cleaves ssDNA and dsDNA in both 3' to 5' and 5' to 3' directions.
- Degrades linear ssDNA and dsDNA leaving intact supercoiled and circular dsDNA.
Description
Exonuclease V is a complex of recombinant RecB, RecC and RecD proteins from E. coli. Enzyme has a helicase and nuclease activity. In the presence of ATP catalyzes exonucleolytic cleavage in both 3' to 5' and 5' to 3' directions to yield 5' phosphooligonucleotides.
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Degradation of linear DNA by Exonuclease V (RecBCD) A&A compared to a competitor enzyme.
/27_AA_Exonuclease_V_RecBCD%20Cropped-1280x720.webp "Exonuclease V (RecBCD)")