Advantages of the product
- Fast, 5-minute procedure
- Up to 10 times higher yield compared to traditional ligation methods
The Liga5™ kit contains a T4 phage DNA Ligase that catalyzes the formation of a phosphodiester bond between juxtaposed 5’-phosphate and 3’-hydroxyl termini in duplex DNA or RNA with blunt or cohesive end termini. The enzyme repairs single stranded nicks in duplex DNA, RNA or DNA-RNA hybrids but has no activity on single stranded nucleic acids. The T4 phage ligase is purified from an E. coli bacterial strain carrying a plasmid with cloned gene g30 of bacteriophage T4.
The Liga5™ kit improves on average 10 times the ligation efficiency of blunt-ended nucleic acids and up to 3 times the ligation of the cohesive ends, compared to traditional DNA ligation.
The Liga5™ ligation mix has no activity on single-stranded nucleic acids.